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PROPRIME™ & PROPRIMEiIndicating
Specimen Collection Cards

Protein & Nucleic Acid Preservation

•Easy Sample Collection

• Nucleic Acids (DNA & RNA) • Protein •

•Ready for processing in minutes!

•Efficient • Reliable • FAST •

• Storage - Ambient or Frozen •

•Also available in an Indicating format for ease of collection of transparent specimens

•Dried Blood Stain (DBS) and Dried Sample\Specimen Stain (DSS) technologies

Gentle
Gentle cell lysis upon contact unlike existing paper matrix product(s) utilizing harsh extraction techniques and methods. PRO™ solves the problem of protein denaturing during the lysis phase of a biological sample.  Cell lysis is the disruption of the cell walls to release the biological components of the cell.  These include DNA, RNA, organalles, and lipids from the cell.  Once the cell has lysed and spilled out its cellular contents, the cell has died.  Cell lysis can occur naturally, in most cases through the drying process.  It can also occur chemically and physically.  Usually when a sample dries the cell and its components lyse onto a matrix or substrate.  Cell lysis fractures the cell and releases its components entrapping the cellular contents within the matrix.   As that occurs, the protein can denature or degrade and become essentially useless.  Other commercially available matrix lysing products that instantly lyse cells are harsh and do not provide a suitable environment for the protein, hence denatures protein structures. 

Protein Preservation
Preservationof proteins – Primary Structures. Proteins are the target with other cellular components (i.e. nucleic acids) trapped in the FP705™ paper.

Nucleic acid preservation
Nucleic acid preservation – primary and secondary structures associated with nuclear proteins.  High molecular weight DNA has been amplified using commercially available PCR kits for human identification and molecular diagnostics.

Kills pathogens

Proven to work with many current Protocols
Applications with current paper matrix collection products using existing DNA processing techniques and protocols with a slight modification or additional wash step.

Automation
Amendable to automation for DNA analysis. Liquid handling systems can be utilized for protein or DNA extractions that include the silica-based extraction chemistries.

 

ProPRIME™ Card Formats

Description

Sample Size
Area

Circles

Specimen
Size***

Style
2 Part unless stated

Overall
Card Size

Qty

ProPRIME™ Classic Card*

3” x 3”

4

125ul (500ul)

Fold over cover

3” x 5”

100/pack

ProPRIMEi™ Classic Card – Indicating**

3” x 3”

4

125ul (500ul)

Fold over cover / Cassette jacket

3” x 5”

100/pack

ProPRIME™ Micro Card

1” Circle

1

125ul

Fold over cover / Cassette jacket

1.5” x 3”

100/pack

ProPRIMEi™ Micro Card – Indicating

1” Circle

1

125ul

Fold over cover / Cassette jacket

1.5” x 3”

100/pack

ProPRIME™ Gene Card*

2.375” x 1”

3

75ul (225ul)

Cassette

2.875” x 2.125”

100/pack

ProPRIMEi™ Gene Card – Indicating**

2.375” x 1”

3

75ul (225ul)

Cassette

2.875” x 2.125”

100/pack

ProPRIME™ TYWN™ Card*

2” x 2.375”

2

125ul (250ul)

Fold over cover

2” x 4.75”

100/pack

ProPRIMEi™ TYWN™ Indicating Card**

2” x 2.375”

2

125ul (250ul)

Fold over cover

2” x 4.75”

100/pack

ProPRIME™ Mini Card*

2.875” x 1.5”

2

125ul (250ul)

Fold over cover

2.875”x1.875”

100/pack

ProPRIME™ Mini Indicating Card**

2.875” x 1.5”

2

125ul (250ul)

Fold over cover

2.875”x1.875”

100/pack

ProPRIME™ Microtiter96™ Sheet*

5” x 3.5”

96

5ul (480ul)

Fold over cover

5” x 5.5”

100/pack

ProPRIME™ Microtiter96™ Sheet Indicating**

5” x 3.5”

96

5ul (480ul)

Fold over cover

5” x 5.5”

100/pack

ProPRIME™ Microtiter384™ Sheet*

5” x 3.5”

384

1.5ul (576ul)

Fold over cover

5” x 5.5”

100/pack

ProPRIME™ Microtiter384™ Sheet Indicating**

5” x 3.5”

384

1.5ul (576ul)

Fold over cover

5” x 5.5”

100/pack

ProPRIME™ Classic Plant Card*

Four -1” Circles

4

25ul (100ul)³

Fold over cover / Cassette jacket

3” x 5”

100/pack

ProPRIME™ Classic DRI™ Card*

Four -1” Circles

4

125ul (500ul)

Fold over cover / Cassette jacket

3” x 5”

100/pack

ProPRIMEi™ Classic DRI™ Indicating Card**

Four -1” Circles

4

125ul (500ul)

Fold over cover / Cassette jacket

3” x 5”

100/pack

ProPRIME™ Micro Plant Card*

1” Circle

1

25ul³

Fold over cover / Cassette jacket

1.5” x 3”

100/pack

ProPRIME™ Micro DRI™ Card*

1” Circle

1

125ul

Fold over cover / Cassette jacket

1.5” x 3”

100/pack

ProPRIMEi™ Micro DRI™ Indicating Card**

1” Circle

1

125ul

Fold over cover / Cassette jacket

1.5” x 3”

100/pack

ProPRIMEi™ Sampact Card - Indicating**

1.25” x .875”

3

75ul (225ul)

Cassette

2.875” x 2.125”

100/pack

*These products will be available by July 29, 2011.
** These products should be available by September 19th, 2011.
*** The standard measurement is based on blood. Specimen size is variable to the actual specimen applied. As well, if plant homogenate is used, it will be approximately 20% of blood.

  Customization is available in printing, barcoding and card formats.

Classic Card -This card has four sample areas for application of up to 500 µl specimen or 100 µl plant homogenate per card. The Classic is excellent for multiple sample collection of the same specimen or collecting of multiple samples on a single card. Its design with a fold over cover helps to protect the specimen area before and after the sample collection. This format & name are consistent and used by both Fitzco and GE/Whatman for the 4 circle card. Each circle is 1 inch in diameter. Each circle will hold around 125ul of whole blood sample or 25 µl plant homogenate . It was named as the “Classic” as it was the first commercial specimen (DBS) card introduced by Fitzco in 1991. This was seven years prior to Whatman purchasing Fitzco for the FTA® and Omni® Swab technologies. This card is the Original Classic Fitzco Card. The Fitzco Card got its name in the 90's during a famous trial in the LA area of California. The lawyers didn’t know what to call it and the manufacturer had never named it beyond the DNA card. They coined the phrase "Fitzco Card", since the printing on the card was Fitzco and it was a DNA card. Prior to Whatman purchasing Fitzco, the card was renamed the "Classic" as the Micro and Gene Cards were released.

The FITZCO® Classic Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cul tured cells, etc.

Micro Card- This card is with a fold over cover. The cover helps to protect the specimen area before and after the sample collection. This type and name of the card is used by both Fitzco and GE/Whatman for the 1 circle card, single sample. The sample area is a 1 inch diameter circle. It will hold around 125ul of whole blood sample or 25 µl plant homogenate per card. Sample punching is easy and consistent for sample analysis. The covers are designed for protection as well to help the sample dry. The hard jacket surrounding the DNA/Specimen paper gives the card rigidity for specimen collection and processing. The Fitzco cards have been in use for over 20 years in both frozen and ambient storage. The products have proven long term storage capabilities. They are also perfect for collection and use immediately.

The FITZCO® Micro Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

Mini Card- This card has two sample areas for application of up to 250 µl specimen or 50 µl plant homogenate per card. This is a fold over cover type card. This type and name of the card is used by both Fitzco and GE/Whatman for the 2 circle card. Each circle is 1 inch in diameter. It will hold around 125ul of sample.

The FITZCO® Mini Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

TWYN™ Card- This card has four sample areas for application of up to 500 µl specimen or 100 µl plant homogenate per card is a card with a fold over cover. This card is unique to Fitzco It has a 2 circle card format but is a larger card. Each circle is 1 inch in diameter. It will hold around 125ul of whole blood sample or 25 µl plant homogenate per circle

The FITZCO® TWYN™ Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

Gene Card- Cassette style Card. It has three sample circles that are only 75ul each it are roughly 2/3” in size. The whole card can collect up to 225 µl whole blood or 30 µl plant homogenate. Used in an automated settings. This format looks like a long slide. It also uses a heavy card stock that surrounds the unique specimen paper. It is made from a rigid cardstock that enhances the specimen cards tensile strength.

The FITZCO® Gene Card can be available as indicating cards* for special orders, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

Sampact® Card- This format looks like the 35mm slide. Fitzco first manufactured this type of card in 1996. It uses a heavy card stock that surrounds the unique specimen paper. It is made from a rigid cardstock that enhances the specimen cards tensile strength.

The FITZCO® Sampact Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

 

DRI™- These cards offer the FITZCO® DRYPAK® Film as a breathable cover. It will be available in the Micro and the Classic format. The Classic DRI™ will also have a jacket for ease of collection. You will be able to utilize this product as an in the field collection device. DRI™ is patent pending & copyrighted.

The FITZCO® DRI™ Card will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

Plant Card™- These cards offer the FITZCO® DRYPAK® Film as a breathable cover. It will be available in the Micro and the Classic format. The Classic Plant Card will also have a jacket for ease of collection. You will be able to utilize this product as an in the field collection device. It is patent pending & copyrighted.

 

Microtiter96 Sheet™- This format was developed to be used as a specimen card but utilizing a 96 well microtiter plate footprint and template. It has 96 small 5ul circles. Each circle is in a grid pattern of nine alpha labeled rows by twelve numbered columns. Fitzco created this format in the mid 1990’s and it was called the Micro Sheet. The non-indicating card was later discontinued after the Whatman purchase. The indicating Micro Sheet was renamed the Clone Saver.

The FITZCO® Microtiter96™ Sheet will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc. © The

 

Microtiter384 Sheet™- Similar to the Microtiter96™, this format was developed to be used as a specimen card but utilizing a 384 well microtiter plate footprint and template. It has 384 small ~2ul circles. Each circle is in a grid pattern of 16 alpha labeled rows by 24 numbered columns.

The FITZCO® Microtiter384™ Sheet will be available as indicating cards*, the FITZCO® indicating cards will turn color as when the sample is applied. They will change from blue to white when a sample is applied, indicating the sample has been applied. This card is recommended for clear specimens such as buccal, cultured cells, etc.

 

ProPIRME™ is Patent Pending.

 

The Ensemble Collection System™ (ECS™), the next generation of specimen card technology.

 

DNA Collection Cards: The Fitzco Card

The Fitzco (DNA) Card originated from an adaption of a card designed for the US military in 1991. The "fold over" flap was added to protect the specimen. The flap was also a useful tool in helping the sample dry. Orginally the card had 4 circles. each circle was then serrated into four parts. This helped the technicians to seperate the samples for preperation in the lab. After the hand punches were introduced, the need for serrations was gone. We abandonded the serrations as past of the design.

At this time, the card was simply known as the Fitzco Card. At Fitzco, we actually just called it the "DNA card". It was the OJ trial that actually branded it as the "Fitzco" Card. It was used by many states and labs for identification and evidence processing, as per the OJ Trial(First). We changed the name of the card to "Classic" because the need for diffent designs; Micro, Gene, Mini, etc.

FP705™ Paper is the base of our Ensemble™ and is the indicating line of all of our products.

 

 

Ensemble Collection System™ - ECS™LineFP705™ Specimen Grade PaperLine705™ Specimen CardsLineProPRIME™ Protein/DNA/RNA CardsLineProPRIME™ Protein/DNA/RNA CardsLinePRO™ Protein/DNA/RNA  CardsLineProPRIME™ Protein/DNA/RNA Cards

Have ideas for a new product? - Contact the Fitzco R&D Team

 

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Telephone +1.952.224.2700 Toll Free(North America) 800.367.8760 .............Fax+1. 952.224.2717 Fax Toll Free(N.A.) 877.471.1185.....

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*FTA® is a registered trademark and owned by GEHC. **Omni Swabs is owned by GEHC.

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